ABSTRACT
Objective:To compare the expression levels of candidate genes before and after Shuganjieyu capsule treatment, to analyze their correlation with depression symptoms and cognitive function, and to find and clarify the biomarkers related to the efficacy of Shuganjieyu capsule.Methods:Among 27 patients with mild to moderate depression (MMD), 24 items Hamilton depression rating scale (HAMD-24) was used to assess the severity of depression, Chinese revised Wechsler adult intelligence scale(WAIS-RC) and Chinese revised Wechsler memory scale(WMS) were used to assess cognitive function, and qRT-PCR was used to detect the expression levels of candidate genes in peripheral blood of patients with depression before and after treatment with Shuganjieyu capsule.SPSS 25.0 software was used for statistical analysis, paired t-test, non-parametric test, Spearman correlation analysis and receiver operating characteristic curve were used for data statistics. Results:The symptoms of MMD patients were relieved after Shuganjieyu capsule treatment(HAMD scores: baseline 14.00(9.75, 18.25), 8-week 4.00(2.00, 7.25), Z=-4.462, P<0.01), and the verbal intelligence quotient(VIQ) of WMS was puomoved (VIQ scores: baseline (123.00±10.24), 8-week (128.00±6.77), t=4.372, P<0.01). The level of gene expression brain derived neurotrophic factor(BDNF) (baseline 1.68(0.92, 2.63), 8-week 2.30(1.47, 4.34), Z=-2.781, P=0.005), glial cell derived neurotrophic factor(GDNF) (baseline 0.74(0.31, 1.15), 8-week 0.97(0.50, 1.71), Z=-2.159, P=0.031), 5-hydroxytryptamine receptor 2A(HTR2A) (baseline 0.60(0.39, 1.60), 8-week 0.98(0.44, 2.29), Z=-1.994, P=0.046) and glutamate ionotropic receptor AMPA type subunit 1(GRIA1) (baseline 1.19(0.66, 2.40), 8-week 1.76(0.86, 4.13), Z=-2.756, P=0.006) was up-regulated after treatment.The change rate of BDNF expression were correlated with the score of HAMD-24 ( r=-0.35, P=0.038) and performance intelligence quotient of WMS ( r=0.40, P=0.022). Conclusions:BDNF may be used as a therapeutic marker of Shuganjieyu capsule in the treatment of clinical symptoms and cognitive function of MMD patients, which is used to evaluate the efficacy of antidepressants.
ABSTRACT
Objective@#Resistance to chemotherapy drugs makes ovarian cancer (OC) difficult to treat and ultimately kills patients. Long non-coding RNAs are closely related to carboplatin resistance in OC. In present study, we explored the role of lncRNA X-inactive specific transcript (XIST) on carboplatin resistance in OC. @*Methods@#Cell viability, proliferation, and apoptosis were assessed through 2,5-diphenyl-2H-tetrazolium bromide, colony formation, and flow cytometry assays, respectively. Microtubule-associated protein 1A/1B-light chain 3 expression was evaluated by immunofluorescence assay to analyze the cell autophagy. The interaction of XIST/miR-506-3p or miR-506-3p/forkhead box protein P1 (FOXP1) was analyzed using RNA immunoprecipitation (RIP) and dual-luciferases reporter assays. The function of XIST/miR-506-3p/FOXP1 axis in vivo was further confirmed by tumor xenograft study and immunohistochemistry. @*Results@#The expression of XIST and FOXP1 increased while miR-506-3p decreased in OC and carboplatin resistance cells. XIST silencing repressed the proliferative and autophagic capacities of carboplatin resistance cells while promoted the apoptosis. XIST overexpression led to the opposite results. XIST targeted miR-506-3p and downregulated its expression. MiR-506-3p inhibition facilitated the proliferative and autophagic capacities while suppressed the apoptosis of cells, XIST knockdown reversed these effects. MiR-506-3p bound to FOXP1. XIST knockdown or miR-506-3p overexpression reversed the increase of cell proliferative and autophagic abilities and the decrease of apoptosis rate induced by FOXP1 overexpression. XIST affected autophagy and carboplatin resistance in vivo via regulating the miR-506-3p/FOXP1 axis. @*Conclusion@#XIST knockdown inhibited autophagy and carboplatin resistance of OC through FOXP1/protein kinase B (AKT)/mammalian target of rapamycin pathway by targeting miR-506-3p.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the personality and character of obstructive sleep apnea hypopnea syndrome (OSAHS) patients.</p><p><b>METHODS</b>Subjects, recruited from May 2013 to February 2014, were assigned to the severe OSAHS group (56 cases), mild-moderate OSAHS group (59 cases), and control group (42 cases) on the basis of apnea hyponea index (AHI). Subjects were assigned to the severe hypoxemia group (24 cases), mild-moderate hypoxemia group (91 cases) on the basis of PaO2. The psychological aspects of subjects were assessed by using the Minnesota multiphasic personality inventory (MMPI).</p><p><b>RESULTS</b>Compared between OSAHS group and the control group, differences of 6 clinical scales depression (D), hysteria (Hy), masculinity (Mf), paranoia (Pa), anxiety (A), ego strength (Es) were significant (t value was 2.609, 2.133, -2.294, 2.520, 2.041, 2.675 respectively, all P < 0.05). The scores of OSAHS group were higher than the control group on five clinical scales, depression (D), hysteria (Hy), paranoia (Pa), anxiety (A), ego strength (ES). The scores of OSAHS group were lower than the control group on clinical scale masculinity (Mf). Compared between severe OSAHS group and mild-moderate OSAHS group, differences of 6 clinical scales depression (D), paranoia (Pa), psychasthenia (Pt) anxiety (A), manifest anxiety scale (MAS), dependency (Dy) were significant (t value was 2.460, 2.086, 2.181, 2.121, 2.954, 1.982, respectively). The scores of severe OSAHS group were all higher than the mild-moderate OSAHS group on these six clinical scales. Compared between severe hypoxemia group and the contrast group, differences of 4 clinical scales depression (D), masculinity (Mf), paranoia (Pa), ego strength (Es) were significant (t value was respectively 2.992, -2.221, 2.164, 2.165, all P < 0.05). The scores of severe hypoxemia group were higher than the control group on 3 clinical scales, depression (D), paranoia (Pa), ego strength (ES), and lower than the control group on clinical scale masculinity (Mf). Compared between severe hypoxemia group and mild-moderate hypoxemia group, psychasthenia (Pt) were significant (t value was 1.984). The scores of severe hypoxemia group were higher.</p><p><b>CONCLUSIONS</b>Compared with health people, OSAHS patients have special personality and character. The degree of OSAHS can infect the personality and character of OSAHS patients.</p>
Subject(s)
Humans , Case-Control Studies , Depression , Hypoxia , MMPI , Personality , Sleep Apnea, Obstructive , PsychologyABSTRACT
Objective To analyze serum markers of transfusion,transmitted infections in pre,transfusion patients and investigate the cause of clinical transfusion related infections.Methods HBsAg,Syphilis antibody,Anti-HCV,and Anti-HIV were detected by ELISA in 3380 Cases.Results Among 3380 patients detected,HBV positive,TRUST positive,HCV positive and HIV positive were 378cases(11.3%),3 cases(0.09%),65 cases(1.9%)and 4 cases(0.12%)respectively.Conclusion Through the serology detections in patients before transfusion,We can identify the patient's health state,avoid the medical dispute resulted from the iatrogenic dissemination diseases and eventually protect the benefits between patients and medical personnnels.
ABSTRACT
Aim To screen the express-altered proteins before or after effect of Ecdysterone on HepG_2 cell model of insulin resistance by the strategy of comparative proteomics, which may approach new proves exploring the target of sensitizer.Methods HepG_2 cells were incubated with 5×10~(-7) mol·L~(-1) insulin for 16 h, and glucose consumption was determined. After treatment, the insulin resistant cells were incubated with 10~(-5) mol·L~(-1) Ecdysterone for 24 h.Then glucose consumption contents were determined. The proteins of two groups before and after treatment with Ecdysterone were extracted by lysis buffers. The express-altered proteins were screened by 2-DE technique.Some of them were analyzed by MALDI-TOF-MS mass spectrometry and MS-Fit database.Results 53 express-altered protein spots of insulin resistant HepG_2 cells before and after treated by Ecdysterone were screened by 2-DE technique,in which 35 ones were up-regulated and the others down-regulated, 6 spots of which were analyzed by MALDI-TOF-MS mass spectrometry and MS-Fit database.Conclusion The target of Ecdysterone as a sensitizer involves many proteins and kinases which correlate insulin resistant. These results lay a foundation for further studies on the function of these target proteins.
ABSTRACT
砄bjective: To study the effects of functinal appliances combined with activator headgear in the treatment of Class II division 1 malocclusion with or without high mandibular angle. Methods: 5 cases (10～12 years old) of class Ⅱ division 1 malocclusion with high mandibular angle (FMA≥34?,HMA) and 8 cases (9～12 years old) without the high angle (FMA
ABSTRACT
0.05). IH scores of 15-PGDH in chorion of placenta and fetal membranes in PL were 1.5?0.6,2.3?0.8, respectively, in TL were 2.6?0.8,3.0?0.7, respectively, and in control group were 4.4?1.1, 4.1?1.2,respectively. IH scores in PL and TL were obviously lower than those in control group(P
ABSTRACT
AIM: To study the role of adrenomedullin(AM) in the pathogenesis of hypoxic pulmonary hypertension. METHODS: Rats were exposed to chronic hypoxia for 14 days. After the measurement of the right ventricular systolic pressure (RVSP), the rats were executed. The weight of the right ventricle (RV), the left ventricle(LV) and the ventricular septum(SP) were determined. The ration RV/(LV+SP) was used to express the thickness of RV. In situ hybridization was used for the detection of the expression of AM mRNA in the lung and RV. RESULTS: The RVSP in the hypoxic group was (63.63?3.42) mmHg,which was significantly higher than that in control group [(34.13?3.40) mmHg]. The RV/(LV+SP) in hypoxic group was 0.439?0.039,which was increased obviously when compared with that of control (0.230?0.025). The level of AM mRNA expressed in the RV in the hypoxia group was significantly higher than that in the control group. CONCLUSION: The expression of AM mRNA in RV increased in the hypoxic condition, which suggests that AM may attenuate the inappropriate increase in pulmonary artery pressure.